Analysis of H2-Antagonist Drugs by HPLC Manual

About this manual

The document presents a High-Performance Liquid Chromatography (HPLC) method developed by Udo Huber, an application chemist at Agilent Technologies. The method is designed for the separation and analysis of cimetidine and ranitidine, which are histamine H2-receptor antagonists used to treat gastritis and stomach ulcers.

The procedure utilizes a Zorbax SB-C18 column with UV detection at 225 nm. Key conditions include an autosampler temperature of 4°C to prevent sample decomposition and a flow rate of 1.0 ml/min. The document details the specific mobile phase composition, column dimensions, and equipment setup required for reliable analysis.

What's inside

• Abstract
• Figure 1 Analysis
• Conditions
• Performance Table
• Equipment List
• Columns
• Note on System Reproducibility

Frequently asked questions

What is the recommended autosampler temperature to prevent sample decomposition?

The autosampler temperature should be set to 4°C to avoid decomposition of the samples.

What is the required injection volume for this HPLC analysis?

The injection volume must be 5 ul for this analysis.