This document introduces how to use vapor generation atomic absorption method to analyze arsenic, antimony and selenium in plant materials.
The document details a method for analyzing toxic elements like arsenic and antimony in plant materials using vapor generation atomic absorption spectroscopy. It highlights the use of a digestion procedure that avoids perchloric acid, utilizing a mixture of sulphuric, nitric, and hydrogen peroxide acids instead.
The study reports results obtained using an Agilent AA-1475 instrument equipped with background correction and a VGA-76 vapor generator. The analysis was performed on NBS orchard leaves (No. 1571), demonstrating the technique's ability to achieve results in good agreement with certified values.
| Instrument Model | Agilent AA-1475 |
|---|---|
| Vapor Generator Model | VGA-76 |
| Recorder Model | Agilent 9176 |
| Printer Model | HP 82905A |
| Sample Type | NBS orchard leaves (No 1571) |
| Sample Mass | approximately 0.5 g |
| Digestion Volume | 5 mL concentrated sulphuric |
| Hydrogen Peroxide Volume | 5 mL volume of 30% v/v hydrogen peroxide |
| Nitric Acid Volume | 2 mL of concentrated nitric acid |
| Final Solution Volume | 25 mL |
| Final Acid Concentration | 7 M hydrochloric acid |
| Integration Time | five 3-second integrations |
The digestion procedure uses a mixture of sulphuric, nitric, and hydrogen peroxide acids.
An Agilent AA-1475 with background correction and a VGA-76 vapor generator were used.
The digested mixture was made up to 25 mL to give a final solution in 7 M hydrochloric acid.
The standard additions calibration procedure was necessary for the determination.