This document describes the method of structure elucidation of complex natural product extracts from ginseng, using rapid resolution liquid chromatography and orthogonal time-of-flight mass spectrometry for component separation and accurate mass measurement. The document discusses the use of a rapid resolution liquid chromatography system and an ion trap mass spectrometer for structure elucidation.
This document describes an analytical method for the structure elucidation of ginsenosides found in a complex Asian ginseng root extract. The study utilizes the Agilent 1200 Series Rapid Resolution LC system equipped with RRHT columns to achieve high-resolution separation of the extract's ingredients. Accurate mass measurements are performed using an Agilent 6200 Series orthogonal acceleration time-of-flight mass spectrometer (oaTOF) with an ESI interface.
The application details the specific experimental conditions for both the liquid chromatography and mass spectrometry systems, including solvent gradients, flow rates, and instrument parameters. Results show that the rapid resolution LC system provides superior peak resolution compared to standard systems, facilitating the identification of major constituents such as ginsenosides Rb1, Re, and Rg1 through accurate mass calculation and collision-induced dissociation (CID) experiments.
| Binary Pump Max Pressure | 600 bar |
|---|---|
| Autosampler Delay Volume | 1.4 pL |
| Column Particle Size | 1.8 pm |
| DAD Sampling Rate | 80 Hz |
| MS Data Acquisition Rate | 40 Hz |
| Flow Rate | 0.5 mL/min |
| Column Temperature | 50° C |
| Injection Volume | 10 pL |
| Fragmentor Voltage | 150 V or 300 V |
The binary pump SL has the capability to deliver a pressure of up to 600 bar.
The RRHT column used has a particle size of 1.8 pm.