This document mainly introduces the analysis method of anticoagulant drugs, including experimental conditions, equipment and sample processing methods
This document details a High-Performance Liquid Chromatography (HPLC) method for analyzing warfarin, an anticoagulant drug. The method utilizes a Zorbax SB-C18 column and variable wavelength or fluorescence detection to determine the drug's concentration with high precision.
| Column | 4.6 x 75 mm Zorbax SB-C18, 3.5 um |
|---|---|
| Mobile phase A | 0.025 M KH2PO4 in water (pH = 3 with H2SO4) |
| Mobile phase B | acetonitrile |
| Flow rate | 1.0 ml/min |
| Gradient | 20 % B to 80 % B in 10 min |
| Column compartment temperature | 25 °C |
| Injection volume | 5 ul |
| LOD | 0.1 mg/l |
The method was specifically developed on the Agilent 1100 Series system.
The recommended column is Zorbax SB-C18, 3.5 um, 4.6 x 75 mm.
The limit of detection (LOD) for warfarin is 0.1 mg/l.