This document describes the HPLC separation method of five common sulfa drugs.
This document details a High-Performance Liquid Chromatography (HPLC) method for separating and analyzing five common sulfa drugs: sulfanilamide, sulfadiazine, sulfathiazole, sulfamerazine, and sulfamethazine. The method utilizes a reversed-phase column with UV detection and gradient analysis.
| Column compartment temperature | 50 °C |
|---|---|
| Injection volume | 5 ul |
The method analyzes sulfanilamide, sulfadiazine, sulfathiazole, sulfamerazine, and sulfamethazine.
The method was specifically developed on the Agilent 1100 Series system.
The column compartment temperature should be set to 50 °C.