This document describes the features and characteristics of insulin, oxytocin, and angiotensin I, II, as well as their roles in blood sugar control, uterine contractions, and blood pressure regulation.
This document presents an HPLC method for the separation and analysis of therapeutic peptides, specifically insulin, angiotensin I, angiotensin II, and oxytocin. The method utilizes a reversed-phase column with UV detection and a gradient mobile phase to achieve reliable separation.
| Column | Zorbax SB-C18, 3.5 um, 4.6 x 75 mm |
|---|---|
| Flow rate | 1.0 ml/min |
| Column temperature | 25 °C |
| Injection volume | 5 ul |
| Autosampler temperature | 4°C |
The Limit of Detection (LOD) for Oxytocin is 2.5 mg/l.
The recommended column is Zorbax SB-C18, 3.5 um, 4.6 x 75 mm.