This document presents a method for the analysis of artemisinin using the Agilent 1200SL with Rapid Resolution High Throughput (RRHT) columns and ChromSword software. The authors developed a simple isocratic method with minimal operator interaction and automated method development. The use of sharp, well-resolved peaks improves data quality and simplifies the analysis process.
This document details the development of a simple isocratic High-Performance Liquid Chromatography (HPLC) method for the analysis of artemisinin and its derivatives. The study utilizes the Agilent 1200SL Rapid Resolution LC (RRLC) system equipped with ChromSword software to perform fully automated, unattended method development over a six-hour period. The goal was to achieve sharp, well-resolved peaks for compounds like artemisinin, dihydroartemisinin, artesunate, and artemether using minimal operator interaction.
| Flow rate | 1 mL/min |
|---|---|
| Column temperature | 25 °C |
| Detection wavelength | 220 nm |
| Mobile phase A | 0.1% formic acid in water |
| Mobile phase B | 0.1% formic acid in acetonitrile |
| Minimum resolution achieved | 2.56 |
ChromSword software was used to aid the analysts in choosing the column suited for the analysis.
The fully automated, unattended method development was performed during a six-hour period.