This application note describes the LC-MS/MS method for determining malachite green (MG) and crystal violet (CV) using Pursuit™ XRs.
This document details a method for the simultaneous analysis of malachite green (MG), crystal violet (CV), and their metabolites using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method is designed for testing these compounds in fish and seafood, addressing regulatory requirements such as those from the Japanese Ministry of Health, Labor and Welfare. It highlights the use of triple-quadrupole MS for maximum sensitivity and selectivity.
| Detection Limit | 0.5 ppb |
|---|---|
| Column Type | Pursuit XRs C18 3um, 150mm x 2mm |
| Flow Rate | 0.2 mL/min |
| Column Oven Temperature | 40 °C |
| MS Ionization Method | ESI |
The detection limit for both MG and LMG using this method is 0.5 ppb.
The method uses a Varian 212 HPLC, Varian 320 MS/MS, and Prostar 410 Autosampler.
The sample injection volume is 20 pL.