This application note describes a method for the effective HPLC determination of fentanyl using an ELSD detector.
This document details an HPLC method for the determination of fentanyl, a potent analgesic and anaesthetic. The method utilizes a Varian evaporative light scattering (ELS) detector, which is noted for its superior performance and independence from the optical properties of the compound. The study analyzes fentanyl concentrations ranging from 0.5 to 200 µg/mL.
| Detector | Varian ELSD |
|---|---|
| Nebulizer Temperature | 40 °C |
| Evaporator Temperature | 70 °C |
| Gas Flow | 1.2 SLM |
| Eluent Composition | 60 % 0.25 M Ammonium acetate in water, 40 % Acetonitrile |
| Flow Rate | 0.25 mL/min |
| Injection Volume | 10 µL |
| Limit of Detection | 10 ng on-column loading |
The eluent consists of 60% 0.25 M Ammonium acetate in water and 40% Acetonitrile.
The limit of detection is 10 ng on-column loading.
The flow rate is 0.25 mL/min and the injection volume is 10 µL.