This is an application note on the HPLC analysis of St John's Wort tablets. The herb contains complex chemicals, including hypericin and hyperforin. This article discusses the uses of the product, its chemical components, and the importance of monitoring and characterization.
St. John's Wort contains complex mixtures of chemicals, including hypericin and hyperforin, which are believed to be the main active ingredients. Because standard detection methods like fluorescence or UV require multiple runs or specific optical properties, this document proposes using a Varian evaporative light scattering (ELS) detector as a universal alternative.
The optimum temperature for analyzing the active ingredients hypericin and hyperforin is 50 °C.
The flow rate for the analysis is 1.0 mL/min.
The required injection volume is 20 wL.