Agilent SI-01663 Application Note Removal of Salts prior to Carbohydrate Analysis

This application note describes how to remove salts from samples using StratoSpheres™ PL-MIXED MP cartridges to protect HPLC columns.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 115 KB
Published: 12 June, 2012
Updated: 30 September, 2023
MD5 checksum: C8BEC03776F9A6FB091DF3B5554AB0C7
PDF: Agilent SI-01663 Application Note Removal of Salts prior to Carbohydrate Analysis PDF

About this manual

The document explains that enzymatic hydrolysis of celluloses and hemicelluloses requires buffer solutions, which must be desalinated before High-Performance Liquid Chromatography (HPLC) analysis to protect the column. The note details a method using StratoSpheres PL-MIXED MP SPE cartridges containing strong cation and anion exchange functionality.

What's inside

Introduction
Conditions
Figure 1: Phosphorus Concentration
Figure 2: Carbohydrate Concentration
Table 1: Recovery Results

Specifications

Cartridge Part Number	PL3540-C610
Bed Mass	500 mg / 6.0 mL
Capacity	1.2 mmol (0.6 mmol cations and 0.6 mmol anions)

Frequently asked questions

What is the purpose of removing salts before HPLC analysis?

Samples must be desalinated to protect the column from loaded cations like Pb2+, Ca2+, Na+, and K+.

Which cartridge is recommended for removing buffer ingredients?

The StratoSpheres PL-MIXED MP SPE strong anion and cation exchanger.

What is the recovery rate for glucose?

The recovery rate for glucose is 93%.

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