The document describes the features and characteristics of the E.coli Competent Cells BL21-Gold (DE3) product kit, including its characteristics of BL21(D[3] competent cells, increased protein expression yield and transformation efficiency, and easy obtainment of high-quality plasmid DNA.
The GeneCopoeia U0107A product is a BL21‑Gold (DE3) competent cell preparation supplied with control DNA (pUC19). The cells are engineered for high transformation efficiency and are stored frozen at -80 °C.
The manual describes step‑by‑step procedures for thawing, adding up to 10 ng of plasmid DNA, heat‑shock at 42 °C for 30–60 seconds, recovery in SOC or LB medium, and subsequent incubation. Precautions include keeping cells on dry ice, avoiding repeated freeze‑thaw cycles, and using low‑volume tubes for best results. This manual is written in Japanese.
| Transformation efficiency | 1×10^8 cfu/µg supercoiled DNA |
|---|---|
| Cell type | BL21‑Gold (DE3) |
| Control DNA | pUC19, 10 pg/µL |
Add up to 10 ng of plasmid DNA per transformation.
Incubate the cells at 42 °C for 30 to 60 seconds.