This document describes the features and principles of the All-in-One™ miRNA qRT-PCR Detection Kit, which is used for quantitative detection of mature miRNA.
This manual describes the principle and workflow of the All-in-One™ miRNA qRT‑PCR Detection Kit, which quantifies mature miRNA using a single‑step poly‑A tailing, reverse transcription, and SYBR® Green qPCR detection. It outlines the three core steps—poly‑A polymerase addition, cDNA synthesis with M‑MLV RTase and an Oligo‑dT adaptor, and real‑time PCR amplification.
The document includes related products, a detailed list of kit contents and storage conditions, preparation guidelines, step‑by‑step procedures, example assays, a troubleshooting guide, and the limited‑use license and warranty terms. Safety and RNase‑free handling recommendations are also provided.