All-in-One™ qPCR Mix is a reagent kit for universal quantitative real-time PCR. It uses a high-fidelity hot-start DNA polymerase, optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification of even low-copy genes. The kit can be used with almost all primers and most real-time PCR instruments, which simplifies experimental design.
The All-in-One™ qPCR Mix is a SYBR® Green‑based real‑time PCR reagent that combines a high‑fidelity hot‑start DNA polymerase, optimized buffer, and high‑quality dNTPs for fast, sensitive amplification of low‑copy genes. The kit is supplied in three catalog numbers—AOPR‑0200 (200 reactions), AOPR‑0600 (600 reactions) and AOPR‑1200 (1200 reactions)—and is compatible with most real‑time PCR instruments.
The manual is organized into sections covering a product description, related products, contents and storage, preparation, detailed PCR procedure, an example workflow, troubleshooting guidance, and the limited‑use license and warranty. It also includes reagent component tables, cycling parameters, and safety recommendations for laboratory handling.