RNAzol®RT is a reagent for isolating total RNA and separating large (>200 nt) and small (<200 nt) RNA fragments, with detailed safety and protocol instructions.
About this manual
RNAzol®RT is a phenol‑based reagent designed for efficient extraction of total RNA and for fractionating large (>200 nt) and small (<200 nt) RNA from cells, tissues, body fluids and other biological samples. The manual provides step‑by‑step procedures for sample preparation, phase separation, precipitation, washing, and dissolution, as well as specific protocols for separating large and small RNA fractions.
Safety precautions are emphasized because the reagent contains phenol and guanidine thiocyanate, both corrosive and toxic. Users are instructed to wear gloves and masks, avoid direct contact, and rinse thoroughly in case of exposure. Storage conditions, shelf life, and product identification details are also included. This manual is written in Chinese.
What's inside
- Product Overview
- Safety Precautions
- Sample Preparation
- Phase Separation
- RNA Precipitation
- Washing and Drying
- Dissolution of RNA Pellet
- Large RNA Isolation
- Small RNA Isolation
- Storage and Shelf Life
Specifications
| Product Number | QP020 |
|---|
| Storage Condition | Room temperature |
|---|
| Shelf Life | At least 2 years at room temperature |
Frequently asked questions
What safety precautions are required when handling RNAzol®RT?
RNAzol®RT contains phenol and guanidine thiocyanate, so users must wear gloves and a mask, avoid skin or clothing contact, and rinse eyes or skin with plenty of water for at least 15 minutes if exposure occurs.
How long can RNAzol®RT be stored at room temperature?
The reagent can be stored at room temperature for at least two years.
What centrifuge speed is recommended for the phase‑separation step?
Spin the sample at 10 000 rpm for 15 minutes during phase separation.
Can RNAzol®RT be used for clinical diagnostics?
No, the product is limited to experimental scientific research and is not intended for clinical diagnosis or treatment.