TF-DetectTM Human p53 Activity Assay Kit is a kit for rapid detection and quantification of active p53
The TF-Detect™ Human p53 Activity Assay Kit enables rapid, sensitive detection and quantification of active p53 in a 96‑well format. The kit includes a recombinant human p53 protein standard, antibodies, buffers, and a 96‑well assay plate.
The manual details kit components, storage conditions, reagent preparation, and a step‑by‑step procedure, including washing, antibody incubations, substrate development, and plate reading. It also contains the limited‑use license, warranty information, and an appendix for nuclear extract preparation.
| Detection limit | 0.8 ng per well |
|---|---|
| Reactions per kit | 96 |
| Antibody storage temperature | -20°C |
| Wash buffer pH | 7.8 |
The assay can detect as little as 0.8 ng of human p53 protein per well.
Each kit contains one 96‑well plate for 96 reactions.
Store the p53 antibody at –20 °C; it remains stable for at least six months.