The document describes the features and characteristics of the M-MLV Reverse Transcriptase (RNase H -) kit, including its functionality as a recombinant RNase H-deficient Moloney Murine Leukemia Virus reverse transcriptase and its applications.
This manual provides an overview of the GeneCopoeia C0201A M‑MLV Reverse Transcriptase (RNase H‑) kit, including product description, enzyme activity, purity, and recommended applications such as cDNA library construction and RT‑PCR. It also lists the available package sizes (50 µl, 250 µl, 1 ml) and storage conditions.
The document outlines the experimental workflow for first‑strand cDNA synthesis, storage guidelines, reaction component mixing, and temperature steps. A FAQ section answers common questions about the advantages of RNase H‑deficient enzyme and template RNA choices. This manual is written in Chinese.
| Packaging options | 50 µl, 250 µl, 1 ml |
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Both total RNA and mRNA can be used; the success of the RT reaction depends on RNA purity, not the RNA type.