The document describes the features and characteristics of the E.coli Competent Cells stbl3 TM product kit.
The Stbl3™ E.coli competent cell kit from GeneCopoeia is designed for high‑efficiency transformation of large plasmids, especially lentiviral vectors. It includes control DNA (pUC19, 10 pg/µl) and detailed genotype information for stable cloning.
The manual outlines storage at –80 °C for up to 12 months, packaging details (100 µl × 10 and 100 µl × 1), and a step‑by‑step transformation protocol with ten steps, followed by important handling precautions such as dry‑ice transport and avoiding repeated freeze‑thaw cycles. This manual is written in Chinese.
| Storage temperature | -80°C |
|---|---|
| Storage duration | 12 months |
| Packaging | 100 µl × 10 and 100 µl × 1 |
| Control DNA concentration | 10 pg/µl pUC19 |
| Transformation efficiency | >1×10^8 transformants per µg pUC19 |
| Genotype | F- mcrB mrr hsdS20 (rB-, mB- ) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (Strr ) xyl-5 - leu mtl-1. |
Store the cells at –80 °C and they remain stable for up to 12 months.
Thaw the cells on ice, add 50‑100 µl to a sterile tube, add DNA (≤10 ng), incubate on ice 30 min, heat‑shock at 42 °C for 30‑60 s, return to ice, add pre‑warmed SOC medium to 1 ml, shake at 37 °C for 1 h, plate on agar, and incubate overnight at 37 °C.
Transport with dry ice, keep cells frozen at –80 °C, avoid repeated freeze‑thaw cycles, handle gently to prevent shear, and use appropriate tubes to maintain transformation efficiency.