Lenti-Pac™ HIV Expression Packaging Kit is an optimized recombinant lentivirus packaging kit that allows for the construction of cDNA clones in the HIV genome and their transfer into an HIV genome, thereby generating recombinant HIV viral vectors.
The Lenti‑Pac™ HIV Expression Packaging Kit (Cat. No. HPK‑LvTR‑20/40) is a third‑generation lentiviral packaging system designed for efficient production of recombinant lentivirus in mammalian cells. The kit includes an optimized plasmid mix, a positive‑control eGFP plasmid, EndoFectin™ Lenti transfection reagent, and TiterBoost™ reagent.
The manual details kit contents and storage conditions, required supplemental materials, and a complete workflow: preparing DNA‑EndoFectin complexes, transfecting 293Ta packaging cells, harvesting virus, estimating viral titer by transduction or drug selection, and performing target‑cell transduction. Licensing, warranty, and biosafety information are also provided.
| HIV packaging mix (HPK‑LvTR‑20) | 100 μl (0.5 μg/μl) |
|---|---|
| Optimal EndoFectin Lenti to DNA ratio | 3.0 µl per 1 µg plasmid |
The HPK‑LvTR‑20 kit is supplied for 20 transfections.
Use 3.0 µl of EndoFectin Lenti for each 1 µg of plasmid DNA.