This document describes the usage and advantages of the MethylAffinityTM Methylated DNA Enrichment Kit, which enables rapid purification and enrichment of methylated DNA. It is suitable for methylation status and location studies.
The MethylAffinity™ Methylated DNA Enrichment Kit (Cat.No.MAK‑GCM‑30) provides a GCM‑bead‑based method for rapid enrichment of methylated DNA from up to 30 µg of fragmented genomic DNA. The kit includes GCM™ beads, binding, elution and salt buffers, and control DNA and primers for up to 30 reactions.
The manual outlines the step‑by‑step protocol—bead preparation, DNA binding, washing, and elution—plus optional gradient elution, storage conditions, and a limited‑use license with warranty information.
| Reactions per kit | 30 reactions |
|---|---|
| Maximum DNA input | 30 µg fragmented genomic DNA |
| GCM beads suspension | 25% (v/v) suspension |
| Buffer stability | Stable for at least 12 months |
The whole procedure can be completed in less than 2 hours.
Store the GCM beads at –20 °C.
The kit is supplied for 30 reactions.