All-in-One™ First-Strand cDNA Synthesis Kit is a kit for reliable first-strand cDNA synthesis from all RNA sources, suitable for gene cloning, cDNA library creation and quantitative PCR amplification
The GeneCopoeia All‑in‑One™ First‑Strand cDNA Synthesis Kit provides a reverse transcriptase and reagents for first‑strand cDNA synthesis from any RNA source. It includes M‑MLV Reverse Transcriptase (RNase H‑), RNase Inhibitor, dNTPs, primers and buffers, and is supplied as two catalog options for 20 or 50 reactions.
The manual covers product description, contents, storage conditions, preparation steps, detailed reaction procedure, an example workflow, troubleshooting tips, and the limited‑use license and warranty terms.
| RNase Inhibitor concentration | 25 U/µl RNase Inhibitor |
|---|---|
| dNTP concentration | 25 mM dNTP |
| Oligo(dT) concentration | 60 µM Oligo(dT) |
| Random Primer concentration | 250 µM Random Primer |
| Reaction Buffer | 5X RT Reaction Buffer |
| Recommended storage temperature | -20 °C (stable ≥12 months); aliquots at -80 °C |
| Aliquot storage condition | -80 °C, avoid repeated freeze‑thaw cycles |
Store the kit at –20 °C (stable for at least 12 months) and keep aliquots at –80 °C; avoid keeping it at 4 °C or room temperature.