This manual introduces GeneCopoeia's Lenti-Pac™ FIV Expression Packaging Kit. The kit can be used to optimize the production of recombinant viruses.
This manual describes the Lenti‑Pac™ FIV Expression Packaging Kit (Cat. No. FPK‑LvTR‑20 and FPK‑LvTR‑40) and its components, storage conditions, and required additional materials. It guides users through plasmid preparation, cell culture, transfection with EndoFectin™ Lenti, lentivirus production, and downstream applications such as titer estimation and target‑cell transduction.
The document also includes safety information, a limited‑use license, and warranty terms. All procedures are designed for Biosafety Level‑2 laboratories and are intended for internal research use only.
| FIV packaging mix volume | 100 µl (0.5 µg/µl) per kit |
|---|---|
| eGFP positive control plasmid volume | 25 µl (0.06 µg/µl) |
| EndoFectin Lenti transfection reagent volume | 500 µl (Kit) / 1 ml (optional) |
| TiterBoost viral titer reagent volume | 500 µl (500×) per kit |
Use 3 µl of EndoFectin Lenti per 1 µg of plasmid DNA; this ratio gives optimal transfection efficiency.
The kit meets Biosafety Level‑2 (BSL‑2) requirements as defined by the CDC.