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- 2
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- ZH · JA
- Last added
- 2026-10-01
GeneCopoeia models by category
Featured GeneCopoeia manuals
GeneCopoeia EFM1004-01/EFM1004-02 EndoFectinTM-Max Transfection Reagent User Manual
The EndoFectinTM‑Max transfection reagent is a lipid‑based product designed to deliver nucleic acids into mammalian cells. It works with a wide range of cell lines, including HEK‑293, CHO‑K1, Hep G2, Hela, MCF‑7, NIH/3T3 and A549, and remains effective in the presence of serum.
The manual provides storage conditions (4 °C–8 °C, sealed, stable for at least 12 months), quality‑control results (≥95 % eGFP expression in HEK‑293 cells), detailed protocols for both transient and stable transfection, recommended DNA‑to‑reagent ratios for various culture vessels, and safety notes for laboratory use. This manual is written in Chinese.
Covers: Product Overview · Quality Control · Precautions · Materials Required · Condition Optimization · Transient Transfection Procedure
GeneCopoeia RNAzol®RT RNA Isolation Reagent User Manual
RNAzol®RT is a phenol‑based reagent designed for efficient extraction of total RNA and for fractionating large (>200 nt) and small (<200 nt) RNA from cells, tissues, body fluids and other biological samples. The manual provides step‑by‑step procedures for sample preparation, phase separation, precipitation, washing, and dissolution, as well as specific protocols for separating large and small RNA fractions.
Safety precautions are emphasized because the reagent contains phenol and guanidine thiocyanate, both corrosive and toxic. Users are instructed to wear gloves and masks, avoid direct contact, and rinse thoroughly in case of exposure. Storage conditions, shelf life, and product identification details are also included. This manual is written in Chinese.
Covers: Product Overview · Safety Precautions · Sample Preparation · Phase Separation · RNA Precipitation · Washing and Drying
GeneCopoeia miProfile miRNA qPCR Primer HmiRQP90 21 User Manual and Validation Report
The miProfile miRNA qPCR Primer is a set of upstream primers designed for specific detection of mature miRNA by qPCR. It is intended to be used together with GeneCopoeia's All‑in‑One miRNA qRT‑PCR Detection Kit for research‑only quantitative analysis.
The document includes product information, recommended reagents, detailed usage procedures (centrifugation, dilution to 2 µM, final 0.2 µM in the reaction), application notes, and a validation report showing experimental setup on a Bio‑Rad iQ5 Real‑Time PCR system with RNA extraction, reverse transcription, and qPCR results. This manual is written in Chinese.
Covers: Overview · Product Information · Recommended Reagents · Usage Procedure · Application · Validation Report
GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
This manual describes the patented cationic polymer EndoFectin™‑CHO, designed for high‑efficiency transfection of CHO cells even in the presence of serum. It outlines the reagent’s advantages, such as superior transfection efficiency, high protein expression, serum‑compatible media, low cytotoxicity, and ease of use.
Detailed procedures cover cell preparation, DNA‑reagent complex formation, transient transfection steps for various culture vessels, and stable transfection workflow including selection of resistant clones. Storage conditions, quality control data, and practical tips for optimizing transfection are also provided. This manual is written in Chinese.
Covers: Product Overview · Composition and Storage · Quality Control · Precautions Before Experiment · Transient Transfection Procedure · Stable Transfection Procedure
Latest GeneCopoeia manuals
GeneCopoeia EFM1004-01/EFM1004-02 EndoFectinTM-Max Transfection Reagent User Manual
User Manual 3 pages · 204 KB · ZHUser manual for GeneCopoeia EndoFectinTM-Max transfection reagent (EFM1004‑01/EFM1004‑02) with storage, handling, and protocol details.
GeneCopoeia RNAzol®RT RNA Isolation Reagent User Manual
User Manual 2 pages · 197 KB · ZHRNAzol®RT is a reagent for isolating total RNA and separating large (>200 nt) and small (<200 nt) RNA fragments, with detailed safety and protocol instructions.
GeneCopoeia miProfile miRNA qPCR Primer HmiRQP90 21 User Manual and Validation Report
User Manual 8 pages · 440 KB · ZHThe GeneCopoeia miProfile miRNA qPCR Primer (catalog HmiRQP90 21) manual provides usage instructions, validation data, and recommended reagents for…
GeneCopoeia TAK-P53-196 Manual
TF-DetectTM Human p53 Activity Assay Kit is a kit for rapid detection and quantification of active p53
GeneCopoeia MAK-GCM-30 user manual
This document describes the usage and advantages of the MethylAffinityTM Methylated DNA Enrichment Kit, which enables rapid purification and enrichment of methylated DNA. It is suitable for methylation status and location studies.
GeneCopoeia HPK-LvTR-20/40 user manual
Lenti-Pac™ HIV Expression Packaging Kit is an optimized recombinant lentivirus packaging kit that allows for the construction of cDNA clones in the HIV genome and their transfer into an HIV genome, thereby generating recombinant HIV viral vectors.
GeneCopoeia FPK-LvTR-20/40 user manual
This manual introduces GeneCopoeia's Lenti-Pac™ FIV Expression Packaging Kit. The kit can be used to optimize the production of recombinant viruses.
GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
User Manual 2 pages · 495 KB · ZHThe GeneCopoeia EndoFectin™-CHO reagent manual provides product overview, storage guidelines, and step‑by‑step transient and stable transfection protocols.
GeneCopoeia AOMD-Q020/Q050 user manual
This document describes the features and principles of the All-in-One™ miRNA qRT-PCR Detection Kit, which is used for quantitative detection of mature miRNA.
GeneCopoeia AOPR-0200/0600/1200 user manual
All-in-One™ qPCR Mix is a reagent kit for universal quantitative real-time PCR. It uses a high-fidelity hot-start DNA polymerase, optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification of even low-copy genes. The kit can be used with almost all primers and most real-time PCR instruments, which simplifies experimental design.
GeneCopoeia AORT-0020/0050 user manual
All-in-One™ First-Strand cDNA Synthesis Kit is a kit for reliable first-strand cDNA synthesis from all RNA sources, suitable for gene cloning, cDNA library creation and quantitative PCR amplification
GeneCopoeia C0201A M-MLV Reverse Transcriptase (RNase H-) User Manual
User Manual 2 pages · 407 KB · ZHUser manual for GeneCopoeia C0201A M‑MLV Reverse Transcriptase (RNase H‑) detailing storage, usage, and reaction protocols.
GeneCopoeia U0107A Competent Cells User Manual
User Manual 2 pages · 356 KB · JAUser manual for GeneCopoeia U0107A competent cells, providing preparation, transformation, and storage instructions.
GeneCopoeia All-in-OneTM Q-PCR Primer Array User Manual
User Manual 7 pages · 478 KB · ZHInstruction manual for GeneCopoeia's All-in-OneTM Q‑PCR Primer Array, a ready‑to‑use 96‑well qPCR kit for gene expression analysis.
GeneCopoeia u10111A Manual
User Manual 2 pages · 383 KB · ZHThis GeneCopoeia manual describes storage, contents, genotype, transformation efficiency, and step‑by‑step use of JM109 E.coli competent cells.
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in GeneCopoeia manuals
What temperature should EndoFectinTM‑Max be stored at?
Store the reagent at 4 °C to 8 °C in a sealed container.
From: GeneCopoeia EFM1004-01/EFM1004-02 EndoFectinTM-Max Transfection Reagent User Manual
What is the transfection efficiency observed in quality‑control testing?
More than 95 % of HEK‑293 cells expressed eGFP after 16 hours.
From: GeneCopoeia EFM1004-01/EFM1004-02 EndoFectinTM-Max Transfection Reagent User Manual
What safety precautions are required when handling RNAzol®RT?
RNAzol®RT contains phenol and guanidine thiocyanate, so users must wear gloves and a mask, avoid skin or clothing contact, and rinse eyes or skin with plenty of water for at least 15 minutes if exposure occurs.
From: GeneCopoeia RNAzol®RT RNA Isolation Reagent User Manual
How long can RNAzol®RT be stored at room temperature?
The reagent can be stored at room temperature for at least two years.
From: GeneCopoeia RNAzol®RT RNA Isolation Reagent User Manual
Which detection kit should be used with the miProfile miRNA qPCR Primer?
It should be used with GeneCopoeia All‑in‑One miRNA qRT‑PCR Detection Kit (Catalog Nos. AOMD‑Q020 or AOMD‑Q050).
From: GeneCopoeia miProfile miRNA qPCR Primer HmiRQP90 21 User Manual and Validation Report
What instrument was used for the validation experiments?
Validation was performed on a Bio‑Rad iQ5 Real‑Time PCR Detection System.
From: GeneCopoeia miProfile miRNA qPCR Primer HmiRQP90 21 User Manual and Validation Report