The GeneCopoeia B0101A Biotin Ligase manual provides storage conditions, product contents, usage instructions, reaction examples, and FAQs.
The B0101A Biotin Ligase kit from GeneCopoeia contains recombinant BirA enzyme (750 U/µl) and buffers for site‑specific biotinylation of AviTag‑fused proteins. The enzyme activates biotin to biotinyl‑5′‑AMP and transfers it to target proteins, enabling downstream applications such as Western blotting.
The manual details product components, purity (>95%), storage guidelines, buffer compositions, a step‑by‑step reaction protocol, precautions, and a FAQ section that addresses common experimental concerns. This manual is written in Chinese.
| Enzyme concentration | 750 U/µl |
|---|---|
| Purity | >95% |
| Long‑term storage temperature | -80 °C |
| Short‑term storage temperature | -20 °C (up to 4 °C for three months >90% activity) |
| Buffer A composition | 0.5 M Bicine, pH 8.3 |
| Buffer B composition | 100 mM ATP, 100 mM MgOAc, 500 µM D‑Biotin |
The Biotin Ligase product has no protease activity; if using crude protein, add an appropriate protease inhibitor to prevent degradation.
No, 0.2 % Tween‑20 has no impact on the reaction.