User manual for GeneCopoeia EndoFectinTM-Max transfection reagent (EFM1004‑01/EFM1004‑02) with storage, handling, and protocol details.
The EndoFectinTM‑Max transfection reagent is a lipid‑based product designed to deliver nucleic acids into mammalian cells. It works with a wide range of cell lines, including HEK‑293, CHO‑K1, Hep G2, Hela, MCF‑7, NIH/3T3 and A549, and remains effective in the presence of serum.
The manual provides storage conditions (4 °C–8 °C, sealed, stable for at least 12 months), quality‑control results (≥95 % eGFP expression in HEK‑293 cells), detailed protocols for both transient and stable transfection, recommended DNA‑to‑reagent ratios for various culture vessels, and safety notes for laboratory use. This manual is written in Chinese.
| Product number | EFM1004-01 / EFM1004-02 |
|---|---|
| Packaging | 1 mL / 3 mL |
| Storage temperature | 4 °C–8 °C (sealed) |
| Shelf life | ≥12 months |
Store the reagent at 4 °C to 8 °C in a sealed container.
More than 95 % of HEK‑293 cells expressed eGFP after 16 hours.
Yes, the reagent works efficiently without removing serum from the culture medium.