The document introduces a high-resolution chiral separation technology for Fluoxetine (Prozac) enantiomers using the ULTRON ES-OVM column
This document details a method for separating and accurately quantitating the R and S chiral forms of Fluoxetine (Prozac). The application demonstrates that the ULTRON ES-OVM column provides exceptional resolution, achieving a separation factor of 3.0 under specific conditions where other columns failed.
The procedure utilizes a mobile phase consisting of ethanol and potassium dihydrogen phosphate at pH 5.5. Key chromatographic parameters include an injection volume of 10 µL, a flow rate of 0.8 mL/min, ambient temperature operation, and UV detection at 225 nm.
The column dimensions are 4.6 x 150 mm.
The procedure requires an injection of 10 pL.