The document describes a method for high-performance liquid chromatography (HPLC) analysis using ion-pair reversed-phase separations combined with electrochemical detection to measure the levels of catecholamines and indoleamines in brain tissue. The method is rapid, selective, and efficient, allowing for analysis of samples in less than 10 minutes per sample. The use of a highly stable column-packing material (Agilent ZORBAX SB-C8, 3.5 µm) ensures unmatched column lifetime, enabling analysis of thousands of samples per column. Samples are directly injected as perchloric acid (0.1N) extracts, eliminating the need for sample workup. This method is suitable for the determination of various biogenic amines neurotransmitters and their metabolites.
This document details a High-Performance Liquid Chromatography (HPLC) method for analyzing neurochemicals such as dopamine, norepinephrine, and epinephrine in mouse brain homogenates. The procedure utilizes ion-pair reversed-phase separations combined with electrochemical detection to achieve rapid and selective analysis.
The rapid and selective analysis is completed in less than 10 minutes per sample.
The separation uses a smaller particle, highly stable, column-packing material (Agilent ZORBAX SB-C8).
The mobile phase flows at 1.5 mL / min.