The document describes the common analysis method for deoxynucleosides and presents a rapid analysis method suitable for LC-MS. The method utilizes Agilent ZORBAX SB-CN column for separation, providing good selectivity and resolution.
The document presents a rapid liquid chromatography (LC) and LC-mass spectrometry (LC-MS) method for the analysis of standard deoxynucleosides. It highlights that while LC allows quantitation, LC-MS enables position confirmation of nucleosides and impurities.
The note details how using shorter columns with 3.5 µm particles instead of longer columns with 5 µm particles can provide very rapid separations suitable for LC-MS without losing resolution. It includes chromatograms demonstrating these improvements.
The shorter column has dimensions of 4.6 x 50 mm.
The method uses a flow rate of 1.0 mL / min.