A comparison study was conducted to evaluate the recovery of two basic amines using two silica-based strong cation exchange (SCX) solid phase extraction (SPE) products. Acidic and neutral impurities were spiked into human plasma to assess the selectivity for amines only. Both Agilent Bond Elut SCX and SampliQ Si-SCX showed comparable and consistent recoveries for the basic compounds used in this study. The use of Bond Elut SCX resulted in a clean extract and high recovery of basic compounds, such as amines.
This document presents a technical overview comparing the performance of two silica-based strong cation exchange (SCX) solid phase extraction products: Agilent Bond Elut SCX and Agilent SampliQ Si-SCX. The study evaluates their ability to recover basic amines, specifically procainamide and chlorpromazine, from human plasma while retaining acidic and neutral impurities.
The results indicate that both cartridges provide comparable recovery rates for the target basic compounds (90-98%). However, the Bond Elut SCX cartridge demonstrated superior performance by offering a cleaner extract with fewer interferences compared to the SampliQ Si-SCX under the tested conditions. The document also details the specific sample preparation scheme and HPLC chromatographic conditions used for analysis.
| Bond Elut SCX Part Number | 12102013 |
|---|---|
| SampliQ Si-SCX Part Number | 5982-2111 |
The Bond Elut SCX offers a cleaner extract relative to the SampliQ Si-SCX under the tested conditions.
Similar recoveries are observed for both cartridges, ranging from 90% to 98%.
The study used an Agilent ZORBAX Rapid Resolution Eclipse Plus C18 column.