This document introduces the advantages of the Varian Evaporative Light Scattering Detector for non-UV chromophore samples, such as those encountered in high throughput screening of combinatorial libraries.
The document highlights the value of the Varian ELSD for analyzing non-UV chromophore samples, such as those found in high-throughput screening of combinatorial libraries. It explains that while UV detection is common for gradient HPLC, it requires compounds to be UV active and have similar extinction coefficients, which is not always the case. The ELSD serves as a universal alternative for detecting solutes without UV chromophores.
| Flow Rate | 2.0 mL/min |
|---|---|
| Column Dimensions | 50 x 2.1 mm |
| Nebulizer Temperature | 85 °C |
| Evaporator Temperature | 50 °C |
| Gas Flow Rate | 1.8 SLM |
It is able to analyze non-UV chromophore samples, such as those encountered in high-throughput screening of combinatorial libraries.
Because for quantification without reference materials, compounds must have similar extinction coefficients and be UV active, which does not always apply.