This document describes the features and characteristics of using Agilent PL aquagel-OH MIXED-H 8μm column for aqueous size exclusion chromatography analysis of polystyrene sulfonate.
This document provides a technical overview for the analysis of hydrophobic compounds, specifically polystyrene sulfonate, using aqueous size exclusion chromatography (SEC). It highlights the stability and high efficiency of the PL aquagel-OH packing material. To prevent ionic interactions between the sample standards and the column packing, the eluent is modified by adding salt. Additionally, a weak organic solvent like methanol is added to further modify the eluent due to the hydrophobic nature of the samples.
| Plate count | >35,000 plates/meter |
|---|---|
| Column dimensions | 300 x 7.5 mm |
| Particle size | 8 um |
| Flow Rate | 1 mL/min |
To counteract ionic interactions between polystyrene sulfonate standards and the column packing.
Because of the hydrophobic nature of the samples, further modification is done by adding a weak organic solvent like methanol.