This document introduces a multiresidue vegetable cleanup method for the determination of six chemicals representing various classes in spinach using Bond Elut Carbon, Carbon/PSA and Carbon/NH2 SPE cartridges.
This technical note evaluates three sorbent variations—Bond Elut Carbon, Bond Elut Carbon/PSA, and Bond Elut Carbon/NH2—for a multiresidue vegetable cleanup method using spinach as a challenging, highly pigmented matrix. The document highlights that these dual-phase SPE products are designed for simplicity, improved analytical performance, and ease of use, featuring ultrapure graphitized carbon particles optimized for adsorbing pigments and small organic residues.
The text details the specific benefits of each product, including pigment removal, excellent recovery of small organics, and compliance with the Japanese Positive List System for pesticide residues. It presents experimental results comparing the performance of these Agilent products against SampliQ alternatives, demonstrating exceptional recoveries and precision for various analytes such as caffeine, tebuthiuron, and warfarin.
| Sorbent Type | Ultrapure graphitized carbon particles |
|---|---|
| Quality Standard | ISO 9001 |
| Column Type | Agilent ZORBAX Rapid Resolution Eclipse Plus C18 |
| Column Dimensions | 4.6 x 150 mm, 3.5 um |
| Detection Wavelength | 254 nm |
Yes, the dual layer Carbon/NH2 Bond Elut is compliant with the Japanese Positive List System for the analysis of pesticide residues in food.
Bond Elut PSA has a significantly higher carbon load and ionic capacity, making it a better choice for polar compounds that retain too strongly on Bond Elut NH2.