This document discusses the methods to avoid column mismatch when analyzing polymers using Agilent PLgel columns. Careful column selection can prevent artifacts when analyzing polydisperse samples.
When low molecular weight individual pore size columns are combined with mixed gel columns, artifacts can occur during the analysis of polydisperse samples. This document explains how careful column selection can prevent these issues.
The text illustrates a specific case involving Polymethylurethacrylate (MW approx. 73,000) analyzed on different PLgel column combinations using THF as an eluent at 1.0 mL/min with RI detection.
Artefacts can occur during the analysis.
The exclusion limit is 60,000 MW.
The exclusion limit is 2,000 MW.