Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF Technical Overview
The document presents a technical overview demonstrating how the Agilent 6540 Accurate-Mass Q-TOF LC/MS System can perform metabolic stability, profiling, and identification in a single experiment. This approach maximizes information obtained from one analysis compared to using separate methods for quantitation and identification.
| Drying Gas Temperature | 350°C |
|---|---|
| Sheath Gas Flow | 12 L/min |
| Capillary Voltage | 4000 V |
| Mass Range (MS and MS/MS) | 100-1000 m/z |
| Acquisition Rate (Combined Mode) | 5 spectra/sec |
| Collision Energy | 28 eV |
The system was operated in an initial MS-only mode at 5 Hz acquisition rate and an automatic MS/MS mode with expected metabolites on the inclusion list.
The quantitative data obtained using the 6540 QTOF showed good correlation with analysis performed on the Agilent 6460 Triple Quadrupole LC/MS System.
The OTOF spectra for buspirone and its metabolites showed mass errors of less than 1 ppm, enabling confident identifications.