This application brief describes the application of CEC to the quantitative analysis of parabenes in body lotion. The method needed minimal sample preparation and provided quantitative data.
This document describes an application of Capillary Electrochromatography (CEC) for the quantitative analysis of parabenes in body lotion. The method utilizes an Agilent Capillary Electrophoresis system equipped with a diode array detector and ChemStation software, employing Agilent CEC C18 columns. The technique requires minimal sample preparation and provides reliable quantitative data.
| Column | Agilent CEC C18 3 um, 250 (335) x 0.1 mm id. |
|---|---|
| Mobile Phase | 80/20 ACN/TRIS 25 mM pH 8.0 |
| Detection | 254/10 nm |
| Injection | 10 s at 5k |
| Run Voltage | 25 kV |
| Temperature | 20 °C |
| Pressure | 8 bar both sides |
| Linear Range | 2 to 20 mg/ml |
The experiments were performed using the Agilent Capillary Electrophoresis system equipped with diode array detector and computer controlled via Agilent ChemStation software.
The response was linear over the range 2 to 20 mg/ml.
Methylparabene was 276 mg/ml, propylparabene was 19 mg/ml, and butylparabene was 49 mg/ml.