This Application Note describes the peak-based purification of 15 samples, which model a part of a combinatorial chemistry library, using the Agilent 1100 Series purification system. The samples were purified using a generic method with a gradient from 5 to 95 % organic phase in relatively short time. The re-analysis of the fractions was done on an analytical HPLC system to check the collected fractions for purity of the desired compounds.
Combinatorial chemistry is frequently used in drug discovery to synthesize large numbers of compounds. This document describes a method for purifying these samples using the Agilent 1100 Series purification system, leveraging the high purity typical of solid-phase synthesis libraries.
The application details a peak-based fraction collection strategy using an UV-visible detector (DAD) or mass selective detector (MSD). A generic HPLC method with a gradient from 5% to 95% organic phase was employed to separate major compounds from byproducts. The collected fractions were subsequently re-analyzed on an analytical Agilent 1100 Series HPLC system to verify purity.
The document describes the peak-based purification of 15 samples that model a part of a combinatorial chemistry library using the Agilent 1100 Series purification system.
The peak detection criteria are a threshold of 150 mAU, a peak slope of 30 mAU/min, and a maximum fraction duration of 0.5 min.