This document describes the features of ZORBAX Bonus-RP, including its unique selectivity and peak shape, which significantly improves resolution between peak 2 and an impurity. In addition, ZORBAX Bonus-RP is a unique polar-linked bonded phase that provides good peak shape, improved quantitation and resolution, long life over a wide pH range, and is suitable for pharmaceutical applications.
This document describes an HPLC application for analyzing the dietary aid mixture known as Fen-Phen, which consists of Phentermine and Fenfluramine. The analysis utilizes a ZORBAX Bonus-RP column to achieve reduced peak tailing and significantly improved resolution compared to standard alkyl-C8 columns.
The procedure details specific chromatographic conditions including an Agilent 1100 HPLC system, a mobile phase of Methanol/Acetonitrile/Phosphate Buffer (pH 7.2), and UV detection at 254 nm.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Mobile Phase Ratio | MeOH: ACN (50:50) |
| Buffer Concentration | 25 mM |
| Detection Wavelength | UV 254 nm |
| Flow Rate | 1 mL/min |
It provides unique selectivity and peak shape that significantly improves resolution between peak 2 (Fenfluramine) and an impurity.
The mobile phase uses a phosphate buffer adjusted to pH 7.2.