Orthogonal methods for protein purification and purity analysis are important to determine with certainty protein identity and purity. The Agilent 1100 Series purification system and the Agilent 2100 bioanalyzer with the Protein 200 Plus LabChip® kit provide an ideal solution that ensures efficient and reliable protein purification and characterization.
This document details an application using the Agilent 1100 Series purification system and the Agilent 2100 bioanalyzer with the Protein 200 Plus LabChip kit to purify and characterize proteins. The 1100 Series system is used for preparative reversed phase (RP) HPLC, while the 2100 bioanalyzer provides orthogonal analysis to determine protein identity and purity.
| Flow rate | up to 100 mL/min |
|---|---|
| Sample capacity | Up to 20 mL |
| Analysis time | less than 30 minutes |
Orthogonal methods provide complementary information on protein characteristics such as molecular weight and hydrophobicity, enabling determination of protein identity and purity with more confidence.
The Agilent 2100 bioanalyzer offers a reliable, fully automated analysis system that does not require tedious separation and staining steps.
The ChemStation A09.03 software is used for system control.