This paper introduces a method for implementing precise time-scaled versions of a method on multiple instrument types using method translation combined with retention time locking (RTL). This method allows the original method to be reused with minimal effort, while optimizing the method for a given sample type or instrument setup.
This document explains how to implement precise time-scaled versions of gas chromatographic methods on multiple instrument types using method translation combined with retention time locking (RTL). It details how this approach allows an original method to be re-used with minimal effort while optimizing it for specific sample types or instrument configurations, thereby extending the utility of the original method.
The text outlines a specific workflow involving the Agilent RTL Pesticide Library method. It describes the steps required to scale a method, including determining a scale factor, calculating inlet pressure and oven temperature adjustments via method translation software, performing RTL calibration runs, and updating the retention time table to match the new scale factor.
RTL is used to calculate the specific inlet pressure required to match retention times precisely after a method has been scaled.
The retention times in the table must be divided by the scale factor using a spreadsheet program like Microsoft Excel.
All systems used for data collection in the examples were equipped with Electronic pneumatics control (EPC).