This is an application note on a drug called pindolol. It is used to lower blood pressure and alleviate depression. The note describes a method for conducting HPLC separation using pindolol and USP method. It also describes a method for conducting HPLC separation using sub-2-micron support particles (ZORBAX SB-CN (L10) columns).
This document describes a method for the rapid analysis of pindolol, a beta blocker used to treat hypertension and potentially depression. The study utilizes High-Performance Liquid Chromatography (HPLC) with ZORBAX SB-CN columns packed with sub-2-micron support particles to achieve faster, more efficient separations compared to traditional USP methods.
| Column Particle Size | 1.8 um |
|---|---|
| Column Dimensions | 4.6 mm x 50 mm |
| Mobile Phase A | 70% pH 5, 0.05 M Na acetate |
| Flow Rate | 1 mL/min |
| Injection Volume | 2 ul |
| Detector Wavelength | 219 nm |
| Maximum Single Impurity | 0.5% |
| Total Impurities Limit | 2.0% |
The USP monograph states that no more than 0.5% of the ratio of impurity peak area to pindolol peak area should be found.
The total of all impurities cannot exceed 2.0%.
No, no adjustments or fine tuning of the method were necessary when converting to 1.8-um particles.