The present application note describes a method for the analysis of small RNAs using the Agilent 2100 bioanalyzer. The method involves the use of the Total RNA 6000 Nano Kit to extract small RNAs, and then the use of the bioanalyzer to separate, detect, and quantify the RNAs. The results showed that the method is highly sensitive and accurate, and is suitable for the rapid and accurate analysis of small RNAs.
This document describes the use of the Agilent 2100 bioanalyzer with the Total RNA 6000 Nano Kit to determine the integrity and concentration of small RNA samples, including 5.8S, 5S RNAs, and tRNAs, extracted from various cell lines. The study compares the performance of the bioanalyzer against traditional techniques like agarose and polyacrylamide gel electrophoresis.
| Detection range | 5 and 500 ng/pl |
|---|---|
| Gel concentration | 15 % polyacrylamide |
| Detection wavelength | 670 nm and 700 nm |
| Electrodes | 16 platinum electrodes |
The Total RNA 6000 Nano Kit was used with the Agilent 2100 bioanalyzer to determine the integrity and the concentration of small RNA samples.
The Agilent 2100 bioanalyzer is superior, as it can detect as little as 5 ng of LMW RNA, whereas agarose gels require at least 500 ng of total RNA for consistent detection.
The analysis included 5.8S, 5S RNAs, and tRNAs, as well as microRNAs in their primitive, precursor, and mature forms.