This article describes the analysis of a complex natural product extract from ginseng. By using LC/MS technology, it is possible to differentiate between different species of ginseng plants and authenticate ginseng products.
The document describes an analytical method using high-performance liquid chromatography (HPLC) coupled with mass spectrometry to analyze complex natural product extracts from ginseng. It focuses on distinguishing between Asian ginseng (*Panax ginseng*) and American ginseng (*Panax quinquefolius*). The study utilizes the Agilent 1200 Series Rapid Resolution LC system equipped with RRHT columns to achieve high-resolution separation of active ingredients, specifically ginsenosides.
The analysis involves detecting and identifying compounds using LC/ESI orthogonal acceleration time-of-flight (oaTOF) and LC/ion trap mass spectrometry. The method allows for the differentiation of regional or biological origins by determining ingredient profiles based on known structural information, thereby providing evidence for the authenticity of ginseng products.
The analysis uses a ZORBAX SB C18 column that is 2.1 mm by 150 mm with a particle size of 1.8 µm.
The system back pressure was typically about 560 bar when using the 1.8 µm column.
The powdered roots must be treated in an ultra sonic bath for 30 minutes in 10 mL methanol before filtration.