Agilent 1100 Series HPLC Solution for High Abundance Protein Depletion Application Note

About this manual

The document presents an automated HPLC workflow designed to deplete six specific high-abundance proteins (albumin, IgG, IgA, haptoglobin, transferrin, and anti-trypsin) from human serum. This process allows for the detection of low-abundance proteins that are otherwise masked by the dynamic range limitations of standard proteomics techniques.

The recommended setup includes an Agilent 1100 Series HPLC system equipped with a quaternary pump, degasser, autosampler, diode-array detector, and fraction collector. The method utilizes specific affinity columns and optimized buffers to capture high-abundance proteins in a single step, collecting low-abundance proteins as flow-through for downstream analysis.

What's inside

• Abstract
• Introduction
• Equipment
• Chromatographic method
• Fraction collection
• Sample processing
• Analysis of proteins
• Results and discussion

Frequently asked questions

Which six high-abundance proteins are removed by the system?

The system removes albumin, IgG, IgA, haptoglobin, transferrin, and anti-trypsin.

What software is recommended for this HPLC solution?

The document recommends using ChemStation A 10.01.