This Application Note demonstrates how the Agilent LC/MS/MS solution can increase productivity through improved performance of the analytical equipment and superior data analysis software that reports relevant parameters directly from the analytical data. Features of the Agilent solution are: Fast LC methods and short cycle times through alternating column regeneration State-of-the-art MS performance Easy-to-learn and easy-to-use acquisition and data analysis software Calculation of PK parameters directly by the data analysis software Software compliant with the regulations for clinical trials.
The document describes a high-throughput analysis method for determining the bio-availability of amoxicillin and clavulanic acid. It utilizes an Agilent 1200 Series Rapid Resolution LC system coupled with an Agilent 6410 triple quadrupole mass spectrometer to achieve cycle times of less than 1.5 minutes.
| Flow rate | 0.8 mL/min |
|---|---|
| Column temperature | 40°C |
| Injection volume | 4 µL |
| Total run time | 1.3 min |
| Amoxicillin calibration range | 10 - 10000 ng/mL |
| Clavulanic acid calibration range | 50 - 5000 ng/mL |
The cycle time was below 1.5 minutes.
Agilent MassHunter workstation software is used to calculate pharmacokinetic values directly.
Amoxicillin is analyzed in positive mode with a precursor ion of 366.2, quantifier ion 114, and qualifier ion 208.