The document discusses the challenge of analyzing minor impurities in partially decomposed samples using liquid chromatography/mass spectrometry (LC/MS) technique. It demonstrates the use of Agilent 1100 Series LC/MSD SL quadrupole mass spectrometer with ChemStation software to improve the detection of impurities. The experiment was conducted using a partially decomposed sample of the bronchodilator salbutamol. The application of data analysis and software tools enhanced the ability to detect impurities.
This document describes an application using the Agilent 1100 Series LC/MSD SL quadrupole mass spectrometer and ChemStation software to analyze minor impurities in a partially decomposed sample of salbutamol. The analysis highlights the challenges of detecting minor components in the presence of chemical noise and high-level components.
| Mobile phase A | 0.1% formic acid in water |
|---|---|
| Mobile phase B | 0.1% formic acid in methanol |
| Flow rate | 0.250 ml/min |
| Source | ESI positive |
| Nebulizer | 25 psig |
| Scan | m/z 100-500 |
Data analysis was performed using the LC/MSD ChemStation software.
A two-step procedure involving background subtract and Overlay base peak chromatogram features is used.
It subtracts the selected background spectrum from each point in the entire MS data set to enhance visualization of impurities.