This document introduces the application of Agilent 1100 quaternary pump equipped with ZORBAX Eclipse-XDB column in food analysis. The column has good chemical stability, and can be used for chromatography separation at intermediate pH values. By shortening the column length and particle size, the analysis time can be shortened, the separation can be maintained, and the solvent consumption can be reduced.
This document details an HPLC method for analyzing FD&C colorants (Yellow #5, Red #40, Blue #1, Red #3) and the preservative propylparaben found in food mixtures. The method utilizes ion-pairing and gradient HPLC to address the wide chemical differences between these compounds.
| Column | ZORBAX Eclipse XDB-C18 Rapid-Resolution (3.5 ym), 4.6 x 50 mm |
|---|---|
| Column Part Number | 935967-902 |
| Mobile Phase A | 0.1% TFA, pH to 4.4 with TEA |
| Gradient | 17 to 100% B / 4min |
| Detection | UV 254 nm |
| Flow Rate | 1 mL/ min. |
| Temperature | ambient |
The analysis is used for food analysis to detect FD&C colorants and preservatives like propylparaben.
The flow rate is set to 1 mL/min.
Detection is performed at 254 nm.