The application note introduces Agilent ZORBAX Eclipse XDB-Phenyl column for flavor and food analysis, which has excellent separation ability and good peak shape.
This document is an application note by Robert Ricker regarding the analysis of aromatics used in the flavor and fragrance industries. It details the separation of five specific compounds: acetophenone, cinnamaldehyde, eugenol, ethyl cinnamate, and p-cymene.
The text highlights the performance of Agilent ZORBAX Eclipse XDB-Phenyl columns for this analysis, noting excellent peak shape for neutral compounds and increased efficiency with smaller particle sizes. It provides specific chromatographic conditions including column dimensions, mobile phase composition, flow rate, and temperature.
| Column | ZORBAX Eclipse XDB-Phenyl, 4.6 x 150 mm (25pm) |
|---|---|
| Mobile Phase | H,0: MeOH, 40:60 |
| UV Detection | 254 nm |
| Flow Rate | 1.0 mL / min. |
| Plate Counts | 13,000 - 18,000 |
Aromatics are used in both the flavor and fragrance industries.
Neutral compounds achieve excellent peak shape on Agilent ZORBAX Eclipse XDB-Phenyl columns.
The specified flow rate is 1.0 mL / min.