This document describes the method of separating vitamins D2 and D3 using the Agilent ZORBAX ODS column. The results show that the non-endcapped column has better retention and resolution than the endcapped column.
This document presents an application for the analysis of fat-soluble vitamins, specifically focusing on the difficult separation of Vitamin D2 and D3. It compares the performance of a classic, non-endcapped Agilent ZORBAX ODS column against its endcapped counterpart.
| Column Dimensions (Classic) | 4.6 x 250mm |
|---|---|
| Column Dimensions (Endcapped) | 4.6 x 150mm |
| Mobile Phase | 75% ACN : 25% MeOH |
| Detection Wavelength | 325 nm for 4 min., 280 nm after |
| Flow Rate | 1.0 mL/min. |
| Injection Volume | 5 pL |
The non-endcapped ZORBAX ODS column outperforms the endcapped ODS in obtaining resolution of vitamins D2 and D3.
Yes, both endcapped and non-endcapped versions are able to separate a wide variety of fat-soluble vitamins, though with differing selectivities.