Aspartame is an artificial sweetener used as a sugar substitute in diet drinks and foodstuffs. This document introduces the analysis method of aspartame and its metabolites in food.
The document details a high-performance liquid chromatography (HPLC) method for analyzing aspartame, phenylalanine, benzyl-3,6-dioxo-2-piperazineacetic acid, and aspartic acid-phenylalanine dipeptide. It is intended for quality control labs in the food industry to test diet drinks and light foodstuffs.
| Column | ZORBAX SB-C18 (4.6 x 75 mm, 3.5 um) |
|---|---|
| Mobile Phase | 85:15, 0.1% TFA:ACN |
| Flow Rate | 1 mL/min |
| Column Temperature | 35°C |
| Detection Wavelength | UV (210 nm) |
Aspartame is used as an artificial sweetener in diet drinks and light foodstuffs, as well as a sugar substitute.
Solid samples must be extracted with water before being injected onto the column.
The column used is 4.6 x 75 mm.