Agilent High-Speed Separation of Pesticide Mixture Application Note

About this manual

This document describes an application for the rapid analysis of a pesticide mixture. It utilizes columns with particle sizes under 5 µm and dimensions up to ten times smaller than standard analytical columns to resolve six specific pesticides in less than two minutes.

The procedure uses an Agilent 1100 HPLC system equipped with a quaternary pump, a ZORBAX Eclipse XDB-C18 Rapid-Resolution (3.5µm) Cartridge-Column (4.6 x 30 mm), and UV detection at 254 nm. The method achieves high resolution while reducing analysis time and solvent consumption.

What's inside

• Application
• Operating Conditions
• Highlights

Frequently asked questions

What is the purpose of using columns with particle sizes under 5 um and dimensions up to ten-times smaller than standard?

These columns are ideal for high-speed analyses that resolve six pesticides in less than two minutes.

Is any modification to the instrument plumbing or flow cell necessary for this application?

No modification to a modern instrument plumbing or flow cell is necessary for this example.

What are the specific operating conditions for the HPLC system, column, mobile phase, detection, flow, and temperature?

The system uses an Agilent 1100 with a ZORBAX Eclipse XDB-C18 Rapid-Resolution (3.5m) Cartridge-Column (4.6 x 30 mm), MeOH: water (60:40) mobile phase, UV 254 nm detection with a 13 uL flow cell, 2mL/ min flow, and ambient temperature.